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Gilbert Greub Lab
Gilbert Greub, MD-PhD, FAMH, PD & MER
++41 (0)21 314 4979
++41 (0)79 556 1795
gilbert.greub@chuv.ch
Research topics
Discovery of new agents of pneumonia
To isolate new bacterial species, we employ an innovative cell culture system that uses free-living amoebae as cellular background. This amoebal co-culture technique selectively grows amoebae-resisting bacteria, i.e. potential pathogens to which humans are frequently exposed (since amoebae are widespread in water), that are highly resistant to water decontamination, and that resist destruction by human macrophages. Once isolated, the role of each new amoebae-resisting species is tested for its pathogenic potential by comparing seroprevalence in patients with and without pneumonia, and by demonstrating the presence of the bacteria in lower respiratory tract samples taken from patients with pneumonia of unknown etiology. This is achieved by developing new diagnostic approaches for each new species (PCR, improved cell culture, immunohistochemistry). For some selected species, we also develop an animal model of pneumonia.
Study of the biology of Chlamydia-like organisms
Chlamydia-related bacteria such as Parachlamydia emerge as novel agents of pneumonia. However, their biology is as yet poorly studied. We aim at understanding how these Chlamydia-like bacteria interact with macrophages and at identifying genes that might be involved in the virulence of these obligate intracellular bacteria. Our work mainly focuses on the intracellular trafficking of Chlamydia-like organisms and on their impact on the host microbicidal effectors.
Selected publications
2009
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1: |
Baud D, Goy G, Gerber S, Vial Y, Hohlfeld P, Greub G.
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Evidence of maternal-fetal transmission of Parachlamydia acanthamoebae.
Emerg Infect Dis. 2009 Jan;15(1):120-1. |
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2: |
Corsaro D, Feroldi V, Saucedo G, Ribas F, Loret JF, Greub G.
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Novel Chlamydiales strains isolated from a water treatment plant.
Environ Microbiol. 2009 Jan;11(1):188-200. |
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3: |
Croxatto A, Greub G.
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Early intracellular trafficking of Waddlia chondrophila in human macrophages.
Microbiology. 2009 Nov 19. [Epub ahead of print] |
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4: |
Dang T, Jaton-Ogay K, Flepp M, Kovari H, Evison JM, Fehr J, Schmid P, Boffi El Amari E, Cavassini M, Odorico M, Tarr PE, Greub G.
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High prevalence of anorectal chlamydial infection in HIV-infected men who have sex with men in Switzerland.
Clin Infect Dis. 2009 Nov 15;49(10):1532-5. |
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5: |
Goy G, Croxatto A, Posfay-Barbe KM, Gervaix A, Greub G.
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Development of a real-time PCR for the specific detection of Waddlia chondrophila in clinical samples.
Eur J Clin Microbiol Infect Dis. 2009 Sep 2. [Epub ahead of print] |
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6: |
Goy G, Greub G.
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Antibiotic susceptibility of Waddlia chondrophila in Acanthamoeba castellanii amoeba.
Antimicrob Agents Chemother. 2009 Mar 30. |
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7: |
Greub G, Kebbi-Beghdadi C, Bertelli C, Collyn F, Riederer BM, Yersin C, Croxatto A, Raoult D.
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High throughput sequencing and proteomics to identify immunogenic proteins of a new pathogen: the dirty genome approach.
PLoS One. 2009 Dec 23;4(12):e8423. |
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8: |
Greub G.
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Parachlamydia acanthamoebae, an emerging agent of pneumonia.
Clin Microbiol Infect. 2009 Jan;15(1):18-28. |
2008
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1: |
Baud D, Jaton K, Bertelli C, Kulling JP, Greub G.
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Low prevalence of Chlamydia trachomatis infection in asymptomatic young Swiss men.
BMC Infect Dis. 2008 Apr 12;8:45. |
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2: |
Baud D, Regan L, Greub G.
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Emerging role of Chlamydia and Chlamydia-like organisms in adverse pregnancy outcomes.
Curr Opin Infect Dis. 2008 Feb;21(1):70-76 |
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3: |
Casson N, Entenza JM, Borel N, Pospischil A, Greub G.
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Murine model of pneumonia caused by Parachlamydia acanthamoebae.
Microb Pathog. 2008 Aug;45(2):92-7. |
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4: |
Casson N, Michel R, Müller KD, Aubert JD, Greub G.
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Protochlamydia neagleriophila as etiologic agent of pneumonia.
Em Infect Dis 2008; 14:168-172 |
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5: |
Casson N, Posfay-Barbe KM, Gervaix A, Greub G.
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New diagnostic real-time PCR for specific detection of Parachlamydia acanthamoebae DNA in clinical samples.
J Clin Microbiol. 2008 Apr;46(4):1491-3. Epub 2008 Jan 30. |
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6: |
Goy G, Croxatto A, Greub G.
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Waddlia chondrophila enters and multiplies within human macrophages.
Microbes Infect. 2008 Apr;10(5):556-62. |
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7: |
Loret JF, Jousset M, Robert S, Saucedo G, Ribas F, Thomas V, Greub G.
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Amoebae-resisting bacteria in drinking water: risk assessment and management.
Water Sci Technol. 2008;58(3):571-577. |
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8: |
Thomas V, Loret JF, Jousset M, Greub G.
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Biodiversity of amoebae and amoebae-resisting bacteria in a drinking water treatment plant.
Environ Microbiol. 2008 Oct;10(10):2728-45. |
2007
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1: |
Baud D, Thomas V, Arafa A, Regan L, Greub G.
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Waddlia chondrophila, a potential agent of human fetal death.
Emerg Infect Dis. 2007 Aug;13(8):1239-43 |
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2: |
Boillat N, Greub G.
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Approche clinique des rickettsioses.
Rev Med Suisse 2007;3:1222-1227. |
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Borel N, Ruhl S, Casson N, Kaiser C, Pospischil A, Greub G.
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Parachlamydia spp. and Related Chlamydia-like Organisms and Bovine Abortion
Em Infect Dis 2007; 13:1904-1907 |
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4: |
Casson N, Entenza JM, Greub G.
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Serological cross-reactivity between different Chalmydia-like organisms.
J Clin Microbiol. 2007 Jan;45(1):234-6. Epub 2006 Oct 25 |
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5: |
Corsaro D, Thomas V, Goy G, Venditti D, Radek R, Greub G
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'Candidatus Rhabdochlamydia crassificans', an intracellular bacterial pathogen of the cockroach Blatta orientalis (Insecta: Blattodea).
Syst Appl Microbiol. 2007 Apr;30(3):221-8. Epub 2006 Aug 24. |
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6: |
Eugster M, Roten CA, Greub G.
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Analyses of six homologous proteins of Protochlamydia amoebophila UWE25 encoded by large GC-rich genes (lgr): a model of evolution and concatenation of leucine-rich repeats.
BMC Evol Biol. 2007 Nov 16;7(1):231 |
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7: |
Goy G, Thomas V, Rimann K, Jaton K, Prod'hom G, Greub G.
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The Neff strain of Acanthamoeba castellanii, a tool for testing the virulence of Mycobacterium kansasii
Res Microbiol. 2007 Feb 20; [Epub ahead of print] |
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8: |
Jaton K et Greub G
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PCR en microbiologie: de l'amplification de l'ADN à l'interprétation du résultat
Rev Med Suisse 2007;3:391-398 |
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9: |
Thomas V, Casson N, Greub G
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New Afipia and Bosea strains isolated from various water sources by amoebal co-culture.
Syst Appl Microbiol. 2007 Nov;30(7):572-9. Epub 2007 Aug 16. |
2006
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1: |
Casson N, Greub G.
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Resistance of different Chlamydia-like organisms to quinolones and mutations in the quinoline resistance-determining region of the DNA gyrase A- and topoisomerase-encoding genes.
Int J Antimicrob Agents. 2006 Jun;27(6):541-4. |
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2: |
Casson N, Medico N, Bille J, Greub G.
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Parachlamydia acanthamoebae enters and multiplies within pneumocytes and lung fibroblasts.
Microbes Infect. 2006 Apr;8(5):1294-300. |
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3: |
Corsaro D, Greub G.
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Pathogenic potential of novel Chlamydiae and diagnostic approaches to infections due to these obligate intracellular bacteria.
Clin Microbiol Rev. 2006 Apr;19(2):283-97. Review. |
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4: |
Jaton K, Bille J, Greub G.
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A novel real-time PCR to detect Chlamydia trachomatis in first-void urine or genital swabs.
J Med Microbiol. 2006 Dec;55(Pt 12):1667-74. |
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5: |
Thomas V, Casson N, Greub G.
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Criblamydia sequanensis, a new intracellular Chlamydiales isolated from Seine river water using amoebal co-culture.
Environ Microbiol. 2006 Dec;8(12):2125-35 |
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6: |
Thomas V, Herrera-Rimann K, Blanc DS, Greub G.
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Biodiversity of amoebae and amoeba-resisting bacteria in a hospital water network.
J Clin Microbiol. 2007 Jan;45(1):234-236. Epub 2006 Oct 25 |
2005
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1: |
Greub G, Collyn F, Guy L, Roten CA.
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A genomic island present along the bacterial chromosome of the Parachlamydiaceae UWE25, an obligate amoebal endosymbiont, encodes a potentially functional F-like conjugative DNA transfer system.
BMC Microbiol. 2004 Dec 22;4(1):48 |
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2: |
Greub G, Desnues B, Raoult D, Mege JL.
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Lack of microbicidal response in human macrophages infected with Parachlamydia acanthamoebae.
Microbes Infect. 2005 Apr;7(4):714-9. Epub 2005 Mar 21. |
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3: |
Greub G, Lepidi H. Rovery C, Casalta JP, Habib G, Collard F, Fournier PE, Raoult D.
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Diagnosis of infectious endocarditis in patients undergoing valve surgery.
Am J Med. 2005 Mar;118(3):230-8 |
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4: |
Greub G, Mege JL, Gorvel JL, Raoult D, Meresse S
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Intracellular trafficking of Parachlamydia acanthamoebae.
Cell Microbiol. 2005 Apr;7(4):581-9. |
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Senn L, Hammerschlag M, Greub G
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Therapeutic approaches to Chlamydia infections.
Expert Opin Pharmacother. 2005 Oct;6(13):2281-90. Review. |
2004
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1: |
Greub G and Raoult D
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Microorganisms resistant to free-living amoebae.
Clin Microbiol Rev. 2004 Apr;17(2):413-33. Review. |
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