Center for Psychiatric Neurosciences                                                                           Français

Proteomics Unit: Service and Research in Psychiatric diseases

Contact information :

Centre de Neurosciences Psychiatriques (CNP)
Site de Cery
CH-1008 Prilly-Lausanne       location plan
Switzerland
Tél. ++41 (0)21 643 63 35/65 08
Fax. ++41 (0)21 643 69 50
E-mail: Beat.Riederer@unil.ch & Beat.Riederer@chuv.ch

Group leader :

Dr. Beat M. Riederer, Ph.D, PD MER

Collaborators :

• Mrs Irène M. Riederer, medical technician
• Mr Nicolas Pochon, apprenti
• Zeinab ElHajj, master student

Description :

The proteomics unit is a service at the CNP open for all research groups at the University of Lausanne and is an extension of the Proteomic Analysis Facility (PAF), UNIL. Its mission is to develop novel technologies for the analysis of protein samples and to facilitate the identification of surrogate markers.

Services consist in :    Anaylsis of protein samples by one- & two-dimensional gel electrophoresis.       Various protein stains (Coomassie blue, Silver-, Sypro Ruby- and Phospho-stains).       Western blots, and analysis of immunoprecipates for associated proteins.       Computer assisted identification of protein differences with Image Master software.       Differential gel electrophoresis of infrared-labeled protein samples for spot identification.       Serial protein labeling in redox proteomics for the identification of oxidized proteins.       Dysregulation of the ubiquitin proteasome system in Alzheimer's disease

At the same time, the research group of Dr. B. Riederer is working on neurodegeneration and investigates metabolic changes in Alzheimer’s disease and Schizophrenia by collaborating with different groups at the CNP. One aspect is protein oxidation and its influence on protein ubiquitination and degradation. We, and others have demonstrated that protein ubiquitination in Alzheimer’s disease is dysfunctioning and may contribute towards protein aggregation and neuronal death. In Schizophrenia the anti-oxidation system of glutathione is not functioning correctly due a genetic variation of a key enzyme and thus may underly the importance of a deficient anti-oxidation defense mechanism in this multifactorial disorder.

Research projects :

Protein oxidation and ubiquitination in neurodegeneration

Recent publications :

To see publications follow this link.

Editorial boards :

• Editorial board of Laboratory Animals, (2007- )
• Guest editor for Special Issue of Brain Research Bulletin on "Neurodegenerative diseases and mechanisms of cell death" (2008/9)
• Section editor Experimental Biology and Medicine (2009-)

Patents:

• Patent No. 08172133.4-1223: Antigenic polypeptides for the detection of chlamydia-related bacteria and test of diagnosis. G. Greub, D. Raoult, C. Kebbi Beghdadi, B. Riederer.
• Patent pending (Waddlia) Antigenic peptides for the detection of Chlamydia-related bacteria and test of diagnosis. G. Greub, D. Raoult, C. Kebbi Beghdadi, B. Riederer.

Teaching tasks :

• Teaching to medical students :
  - Médecine 1^ère année: Module 1.4R :
    Système locomoteur : cours & travaux pratiques  R
  - Médecine  2^me année : Module 2.6 d’Anatomie :
    Bassin et Périnée : cours & travaux pratiques
  - Médecine 2^me année : APP
  - Médecine 2-3^me années
    Démonstrations anatomiques, Système nerveux
    
• Teaching to PhD students in Neuroscience :
  - Module Neuroscience Psychiatrique pour SSP et Biologie
    Neuroanatomie comparée, cours & travaux pratiques
    
• Teaching to students at EPFL :
  - UE H Architecture en mouvement, 1^st year
  - Master of Science in Sport Administration and Technology :
    Introduction to cells, genes, metabolism and anatomy : Travaux pratiques & démonstrations
    
• Teaching at the Lemanic Neuroscience Doctoral School :
  - Module Neurodegeneration pour PhD en neuroscience
    
• Teaching at faculty SSP and in sport
  - Sport Universitaire : Anatomie fonctionnelle
  - Fédération international de Chiropratique du Sport
    Cours : Female pelvis, Needeling, practicals
    
• Continued Education :
  - Sonography and injections, TRB & DocForDoc
  - Pelvic floor anatomy and surgery TEGEA
  - Safety in the animal house, USGEB symposium

Keywords :

Alzheimer's disease, Oxidation, Electrophoresis, Schizophrenia, one- and two-dimensional gel electrophoresis, protein labeling

Local, national and international collaborations :

• Geneviève Leuba (CNP)
• Kim Do (CNP)
• Françoise Schenk (CNP)
• Manfredo Quadroni (PAF, CIG, Lausanne)
• Gilbert Greub (CHUV, Lausanne)
• Costa Bouras (Genève)
• Ingmar Blümcke (Erlangen, Germany)
• Joël Eyer (Anger, France)