Amandine MATHIAS | PhD, Research Associate |
Sylvain PERRIOT | PharmD PhD, Post-Doc |
Mathieu CANALES | Technician |
Larise OBERHOLSTER | MSc, PhD student |
Samuel JONES | MD-PhD student |
Marie GIMENEZ | MSc, research assistant |
Lucas CULEBRAS | MSc, research assistant |
Primarily devoted to the research in Multiple Sclerosis (MS), our Laboratory studies the interaction between the immune response - with a focus on CD8+ T cells - and environmental factors. In particular, we have investigated the mechanism by which Epstein-Barr virus (EBV) might be implicated in the pathogenesis of MS. We have found that EBV-specific CD8+ T cells are dysregulated in the blood and the cerebrospinal fluid (CSF) of MS patients. In order to examine whether EBV-specific CD8+ T cells would cross-react with central nervous system (CNS) cells, we have established a program of human induced pluripotent stem cells (hiPSC). We have thus set up an in vitro human model allowing to study, in a fully autologous way, the interaction between peripheral immune cells and CNS cells. Thanks to this potent tool, several opportunities opened to us and are leading to fruitful collaborations.
We also have a long-standing interest in the immunopathogenesis of progressive multifocal leukoencephalopathy (PML), a disease mediated by the polyomavirus JC (JCV). Our hiPSC technology offers a beautiful opportunity to modelize this complex disease in vitro. Indeed, up to now, there is no animal models of PML and in vitro cultures were done on tumoral CNS cells, thus poorly reflecting the real brain environment.
The Laboratory of neuroimmunology run by Prof Renaud Du Pasquier is linked to other laboratories of research in immunology, in particular the Laboratory of Cellular Immunology and AIDS Immunopathology (Prof. Giuseppe Pantaleo) and the Laboratory of experimental neuroimmunology (Prof Caroline Pot). Lab’s main research topic is the study of the immune- and neuro-pathogenesis of demyelinating illnesses, including:
1. Multiple Sclerosis (MS)
MS is a disease that encompasses two phases: the inflammatory and the neuro-degenerative ones. Whereas major improvements to tame the inflammatory phase have been achieved thanks to the famous disease-modifying therapies (DMTs), MS remains without cure. We believe that only a full understanding of the immunopathogenesis of MS will allow to find a curative (or preventive) treatment. This theme is explored through the following lines of research:
This program opens major perspectives. Indeed, it will be possible to:
2. Progressive multifocal encephalopathy (PML)
JC virus (JCV) is the causative agent of progressive multifocal leukoencephalopathy (PML), a demyelinating disease of the central nervous system (CNS) that results in the widespread formation of lesions across the brain parenchyma 12. JCV is an opportunistic virus that resides in a latent state in the kidneys of more than half of the adult population 13. However, in rare cases of severe immune suppression, the virus establishes infection in the brain where it infects oligodendrocytes and astrocytes. The infection of oligodendrocytes is lytic and results in demyelination, the hallmark of the disease 14. Since there exist no antiviral therapies against JCV, the only way to halt disease progression is to reconstitute an adequate immune response, which is often impossible 15.
To better understand the pathogenesis of PML, our lab is involved in the following lines of research:
Prof. Giuseppe PANTALEO | Laboratory of immunopathogenesis of HIV, CHUV, Switzerland |
Prof. Nicole DEGLON | Laboratory of cellular and molecular neurotherapy, CHUV, Switzerland |
Prof. Caroline POT | Laboratory of experimental neuroimmunology, CHUV, Switzerland |
Dr. Matthias CAVASSINI | Division of HIV/AIDS, Service of infectious diseases, PD MER, CHUV, Switzerland |
Prof. Britta ENGELHARDT | University of Bern, Theodor Kocher Institute, Switzerland |
Prof. Tobias DERFUSS | University of Bern, Switzerland |
Prof. Jens KUHLE Prof. Cristina GRANZIERA | University of Basel, Switzerland |
The different Swiss Centers part of the Swiss HIV Cohort Study |
Prof. David LAPLAUD | INSERM UMR 1064, Nantes, France |
Dr Guillaume MARTIN-BLONDEL Prof. Roland LIBLAU | INSERM U1043, Toulouse, France |
Dr Nicholas SCHWAB Prof. Heinz WIENDL | University Hospital of Münster, Germany |
Prof. Hideaki NISHIHARA | Yamaguchi University, Yamaguchi, Japan |
Prof. Igor KORALNIK | Northwestern Memorial Hospital, Chicago, USA |
Bernard-Valnet R, Koralnik IJ, Du Pasquier R
Ann Neurol. 2021 Dec;90(6):865-873
Bernard-Valnet R, Moisset X, Maubeuge N, Lefebvre M, Ouallet JC, Roumier M, Lebrun-Frenay C, Ciron J, Biotti D, Clavelou P, Godeau B, Du Pasquier RA, Martin-Blondel G
Neurol Neuroimmunol Neuroinflamm. 2022 Jan;9(1)
Perriot S, Canales M, Mathias A, Du Pasquier R
STAR Protoc. 2021 Dec 17;2(4):100902
Bernard-Valnet R, Perriot S, Canales M, Pizzarotti B, Caranzano L, Castro-Jiménez M, Epiney JB, Vijiala S, Salvioni-Chiabotti P, Anichini A, Salerno A, Jaton K, Vaucher J, Perreau M, Greub G, Pantaleo G, Du Pasquier RA
Neurol Neuroimmunol Neuroinflamm. 2021 Jul;8(5)
Nishihara H, Gastfriend BD, Soldati S, Perriot S, Mathias A, Sano Y, Shimizu F, Gosselet F, Kanda T, Palecek SP, Du Pasquier R, Shusta EV, Engelhardt B
FASEB J. 2020 Dec;34(12):16693-16715
Killestein J, Du Pasquier R, Hemmer B
Neurology. 2020 Oct 6;95(14):613-614
Bernard-Valnet R, Pizzarotti B, Anichini A, Demars Y, Russo E, Schmidhauser M, Cerutti-Sola J, Rossetti AO, Du Pasquier R
Eur J Neurol. 2020 Sep;27(9):e43-e44
Du Pasquier RA
Neurol Neuroimmunol Neuroinflamm. 2019 Nov;6(6):e629
Santos GMA, Locatelli I, Métral M, Calmy A, Lecompte TD, Nadin I, Hauser C, Cusini A, Hasse B, Kovari H, Tarr P, Stoeckle M, Fux C, Di Benedetto C, Schmid P, Darling KEA, Du Pasquier R, Cavassini M, Neurocognitive Assessment in the Metabolic and Aging Coh
Open Forum Infect Dis. 2019 Jul;6(7):ofz277
Mathias A, Perriot S, Canales M, Blatti C, Gaubicher C, Schluep M, Engelhardt B, Du Pasquier R
Neurol Neuroimmunol Neuroinflamm. 2017 Nov;4(6):e401
Merienne N, Vachey G, de Longprez L, Meunier C, Zimmer V, Perriard G, Canales M, Mathias A, Herrgott L, Beltraminelli T, Maulet A, Dequesne T, Pythoud C, Rey M, Pellerin L, Brouillet E, Perrier AL, du Pasquier R, Déglon N
Cell Rep. 2017 Sep 19;20(12):2980-2991
Mathias A, Perriard G, Canales M, Soneson C, Delorenzi M, Schluep M, Du Pasquier RA
Mult Scler. 2017 May;23(6):802-809
Bacchetta F, Mathias A, Schluep M, Du Pasquier R
Mult Scler. 2017 Feb;23(2):300-303
Simioni S, Cavassini M, Annoni JM, Métral M, Iglesias K, Rimbault Abraham A, Jilek S, Calmy A, Müller H, Fayet-Mello A, Giacobini E, Hirschel B, Du Pasquier RA
Neurology. 2013 Feb 5;80(6):553-60
Du Pasquier RA, Jilek S, Kalubi M, Yerly S, Fux CA, Gutmann C, Cusini A, Günthard HF, Cavassini M, Vernazza PL, Swiss HIV Cohort Study.
AIDS. 2013 Jan 14;27(2):203-10
Antoniol C, Jilek S, Schluep M, Mercier N, Canales M, Le Goff G, Campiche C, Pantaleo G, Du Pasquier RA
Neurology. 2012 Dec 4;79(23):2258-64
Jilek S, Schluep M, Harari A, Canales M, Lysandropoulos A, Zekeridou A, Pantaleo G, Du Pasquier RA
J Immunol. 2012 May 1;188(9):4671-80
Lysandropoulos AP, Jaquiéry E, Jilek S, Pantaleo G, Schluep M, Du Pasquier RA
J Neuroimmunol. 2011 Apr;233(1-2):240-4
Guarda G, Braun M, Staehli F, Tardivel A, Mattmann C, Förster I, Farlik M, Decker T, Du Pasquier RA, Romero P, Tschopp J
Immunity. 2011 Feb 25;34(2):213-23
Simioni S, Cavassini M, Annoni JM, Rimbault Abraham A, Bourquin I, Schiffer V, Calmy A, Chave JP, Giacobini E, Hirschel B, Du Pasquier RA
AIDS. 2010 Jun 1;24(9):1243-50
Jaquiéry E, Jilek S, Schluep M, Meylan P, Lysandropoulos A, Pantaleo G, Du Pasquier RA
Eur J Immunol. 2010 Mar;40(3):878-87
Jilek S, Jaquiéry E, Hirsch HH, Lysandropoulos A, Canales M, Guignard L, Schluep M, Pantaleo G, Du Pasquier RA
Lancet Neurol. 2010 Mar;9(3):264-72
Vulliemoz S, Lurati-Ruiz F, Borruat FX, Delavelle J, Koralnik IJ, Kuntzer T, Bogousslavsky J, Picard F, Landis T, Du Pasquier RA
J Neurol Neurosurg Psychiatry. 2006 Sep;77(9):1079-82
Du Pasquier RA, Stein MC, Lima MA, Dang X, Jean-Jacques J, Zheng Y, Letvin NL, Koralnik IJ
J Neuroimmunol. 2006 Jul;176(1-2):181-6
Du Pasquier RA, Schmitz JE, Jean-Jacques J, Zheng Y, Gordon J, Khalili K, Letvin NL, Koralnik IJ
J Virol. 2004 Sep;78(18):10206-10
Du Pasquier RA, Kuroda MJ, Zheng Y, Jean-Jacques J, Letvin NL, Koralnik IJ
Brain. 2004 Sep;127(Pt 9):1970-8
Du Pasquier RA, Kuroda MJ, Schmitz JE, Zheng Y, Martin K, Peyerl FW, Lifton M, Gorgone D, Autissier P, Letvin NL, Koralnik IJ
J Virol. 2003 Nov;77(22):11918-26
Du Pasquier RA, Corey S, Margolin DH, Williams K, Pfister LA, De Girolami U, Mac Key JJ, Wüthrich C, Joseph JT, Koralnik IJ
Neurology. 2003 Sep 23;61(6):775-82